Spot Test

Stock solution: 1% potassium iodide in distilled water.

1. In a test tube a few drops (~200 ^l) of stock potassium iodide solution is added (~6-7 drops from a transfer pipette).

2. Add about 100 ^l of urine specimen (~3-4 drops) suspected of PCC adulteration.

3. Add two drops of 2 N hydrochloric acid. Immediate release of iodine from the colorless potassium iodide solution is seen if PCC is present in the urine. Shaking of this solution with n-butanol results in the transfer of iodine in the organic phase. If no nitrite is present, the potassium iodide solution remains colorless. There is no interference from high glucose or ketone bodies if present in the urine.

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