Spot Test

Wu et al. also described the protocol for detection of PCC in urine using spot tests. The indicator solution contains 10 g/L of 1,5-diphenylcarbazide (DPC) in methanol. The indicator detects the presence of chromium ion and is colorless when prepared.

Two drops of indicator solution is added to 1.0 mL of urine. If a reddish-purple color develops, the test is positive (24). Paul et al. also used DPC for detection of PCC in urine. When this reagent is added, a red-violet color appears immediately if PCC is present. The chromium-DPC complex shows a characteristic absorption peak at 544 nm and a shoulder peak at 575 nm. The ratio of absorption can be used to detect the presence of PCC as chromium in urine, and concentration of chromium can be estimated by measuring absorption at 544 nm, with a linear association between concentrations of 0.5 and 20 ^g/mL (25).

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