Spot Test

Wu et al. also described the protocol for detection of PCC in urine using spot tests. The indicator solution contains 10 g/L of 1,5-diphenylcarbazide (DPC) in methanol. The indicator detects the presence of chromium ion and is colorless when prepared.

Two drops of indicator solution is added to 1.0 mL of urine. If a reddish-purple color develops, the test is positive (24). Paul et al. also used DPC for detection of PCC in urine. When this reagent is added, a red-violet color appears immediately if PCC is present. The chromium-DPC complex shows a characteristic absorption peak at 544 nm and a shoulder peak at 575 nm. The ratio of absorption can be used to detect the presence of PCC as chromium in urine, and concentration of chromium can be estimated by measuring absorption at 544 nm, with a linear association between concentrations of 0.5 and 20 ^g/mL (25).

Dealing With Asthma Naturally

Dealing With Asthma Naturally

Do You Suffer From ASTHMA Chronic asthma is a paralyzing, suffocating and socially isolating condition that can cause anxiety that can trigger even more attacks. Before you know it you are caught in a vicious cycle Put an end to the dependence on inhalers, buying expensive prescription drugs and avoidance of allergenic situations and animals. Get control of your life again and Deal With Asthma Naturally

Get My Free Ebook


Post a comment