Spot Test 1

Stock solution: 2% potassium permanganate in distilled water and 2 N hydrochloric acid.

1. In a test tube add about 200 ^L of stock potassium permanganate solution (~6-7 drops from a transfer pipette).

2. Add about 100 ^l of urine specimen (~3-4 drops) suspected of nitrite adulteration.

3. Add two drops of 2N hydrochloric acid.

The pink permanganate solution turns colorless with effervescence immediately after addition of hydrochloric acid if nitrite is present. This is due to reduction of heptavalent manganese ion of potassium permanganate by nitrite. The presence of very high glucose in urine (glucose > 1000mg/dL) and ketone bodies may cause false positive. However, it takes approximately 2-3 min for the solution to turn colorless. On the other hand, if nitrite is present, the solution turns colorless immediately.

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