Although immunoassays are widely used for determination of therapeutic drug concentrations in clinical laboratories, other analytical techniques such as GC, HPLC, GC/MS, and HPLC combined with tandem mass spectrometry also have applications for TDM. Immunoassays can provide a result rapidly because of ease of operation, but these assays also suffer from specificity because of cross-reactants. Moreover, immunoassays are not available for all drugs monitored in clinical laboratories. Therefore, HPLC, GC/MS, and especially HPLC combined with tandem mass spectrometry are essential for TDM especially in reference laboratories and hospital laboratories of academic medical center and referral hospitals.

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