Conclusion

Despite limitations, enzyme-based immunoassays are used for rapid determination of alcohol concentrations in clinical laboratories, but for forensic application, GC or GC/MS should be the method of choice. Breath analysis is a rapid way to identify a subject with alcohol intoxication, but this test also suffers from limitation. Alcohol can also be measured indirectly by using osmolar gap; but direct measurement of alcohol in serum or whole blood is the state of art practice. For legal alcohol, caution should be taken with regard to methodology and chain of custody.

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