Even with the discovery of many new antidepressants, TCAs are still widely used and are a common cause of morbidity and mortality. In overdose situations, comprehensive drug-screening methods commonly involve screening of TCAs by using immunoassays. There are a number of drugs that interfere with immunoassays for TCAs and can cause false-positive results and may lead to misdiagnosis and mismanagement of a patient. Knowledge of the drugs that interfere with TCA assays and methods to eliminate these interferences may be very useful in correct diagnosis. Chromatographic methods, although cumbersome and not readily available, are relatively free from interferences. The role of therapeutic drug monitoring in analysis of TCAs and many non-TCAs is unequivocal.

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