Conclusion

Interference from heterophilic antibody to an immunoassay resulting in a false-positive or false-negative result is more frequently observed with immunometric or sandwich assays than in competition assays. Because competition assays are commonly used in assays for drugs in TDM, interference of heterophilic antibody is only infrequently encountered in TDM. Sample pretreatment with blocking agents may eliminate this interference. Alternatively monitoring free drug concentration in protein-free ultra-filtrate eliminates this interference.

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