Short to intermediate-acting (amobarbital, butalbital, pentobarbital, and secobarbital) barbiturates are most commonly abused, whereas phenobarbital, a long-acting barbiturate used as an antiepileptic, is rarely abused. Several immunoassays for barbiturates are available: EMIT, FPIA, CEDIA, KIMS, and RIA. Some of the assays can be run at 200 or 300ng/ml cutoffs, with secobarbital as the calibrator. Most of these assays have sufficient cross-reactivity to detect butalbital and amobarbital also (25). Immunospeci-ficity for other barbiturates such as phenobarbital and pentobarbital varies with the assays.

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