Atmospheric Steam Sterilization

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Steam Injection Begins

Substrate Temperature. 104° F

220 205 130 175 160 145 130 115 V00 85 70

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Steam Injection Begins

Substrate Temperature. 104° F

Figure 138. Profile of Atmospheric Steam Sterilization method for sterilizing sawdust-based substrates.

or freshly laundered clothes.

Many spawn producers autoclave on one day, allow the vessel to cool overnight, and open the vessel the next morning. Depending on the mass of the autoclaved material, 12-24 hours may pass before the internal temperatures have fallen below 100° F. (38° C.), the minimum plateau for successful inoculations. Some of the better equipped spawn producers have large laminar flow hoods, even laminar flow "walls" in whose airstream the sterilized mass cools prior to inoculation.

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