Mm

Perspex cylinder

Artificial CSF (aCSFl

Soft paraffin wax n 5 mm n

Perspex cylinder

Soft paraffin wax

Dura .mater mater

Figure 4.5 The cortical cup. The cup size obviously varies with the animal on which it is used. For the rat, the overall diameter is 6-7 mm and a typical flow rate is 50 ^l/min

Dura .mater mater

Cerebral cortex

Figure 4.5 The cortical cup. The cup size obviously varies with the animal on which it is used. For the rat, the overall diameter is 6-7 mm and a typical flow rate is 50 ^l/min been adopted in studies of peptide and purine release as well (e.g. Phyllis, Smith-Barbour and O'Regan 1996). The cup comprises a 'well', constructed above a small hole drilled in the skull, which contains aCSF. Transmitters released from neurons near the surface of the brain accumulate in the cup and their concentration in the aCSF can be used as a crude index of release rate. The importance of this technique is that it enabled, for the first time, the monitoring of transmitter release in freely moving animals.

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