The basis of the photometry method is a comparison of the extent of the absorption of radiant energy at a particular wavelength in a solution of the test material with that in series of standard solutions. Filter photometers are suitable for routine methods that do not involve complex spectra287. In practical laboratory work the photometric micromethod was developed for determination of sparteine, lupanine, lupinine, hydroxylupanine and angustifoline282. This method, tested on model solutions, is suitable for the determination of alkaloids in vegetal material of very low alkaloid content.
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