High performance liquid chromatography

High performance liquid chromatography (HPLC) is a modern application of liquid chromatography. High performance liquid chromatography guarantees a high sensitivity and, at the same time, this technique has its gas analogue. The principle of HPLC is the same as that of liquid chromatography (LC), liquidsolid chromatography (LSC) and liquid-liquid chromatography (LLC). High performance liquid chromatography is the most recent technique.

The stationary phase may be a solid or liquid on a solid support. The mechanisms responsible for distribution between phases include surface absorption, ion exchange, relative solubilities and steric affects289'290'291'292. High performance liquid chromatography is a useful method for quinolizidine alkaloid analysis, especially when pure standards are available4. This method was recently used for alkaloid metabolite extraction and analysis308 309. A simple reversed-phase liquid chromatographic method has been developed for the simultaneous quanti-tation of four anticancerous alkaloids vincristine, vinblastine, and their precursors catharanthine and vindoline using a specific HPLC column310.

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